Initiation of RNA synthesisin vitroby vesicular stomatitis virus: single Internal initiation in the presence of aurintricarboxylic acid and vanadyl ribonucleoside complexes
نویسندگان
چکیده
منابع مشابه
Initiation of RNA Synthesis in Vitro by Vesicular Stomatitis Virus
The ATP requirement during RNA transcription by the RNA-dependent RNA polymerase associated with purified vesicular stomatitis virus was studied during chain initiation and chain elongation steps. Initiation of transcription in vitro has an apparent K,,, for ATP of approximately 500 PM, whereas the apparent Km for ATP during chain elongation is almost identical with those for the other three ri...
متن کاملInhibition by aurintricarboxylic acid and polyethylene sulfonate of RNA transcription of vesicular stomatitis virus.
The in vitro activity of the ribonucleoprotein-dependent RNA transcriptase of vesicular stomatitis virions was found to be completely inhibited by low concentrations of aurintricarboxylic acid (ATA) and polyethylene sulfonic acid (PES) when these inhibitors were added before the start of the RNA polymerase reaction. However, if RNA synthesis was allowed to occur before ATA or PES was added, RNA...
متن کاملInitiation and replication of vesicular stomatitis virus genome RNA in a cell-free system.
A system for studying the in vitro replication of the RNA genomes of both wild-type vesicular stomatitis virus (VSV) and its defective interfering particle MS-T has been developed. After lysolecithin treatment of cells infected with VSV or VSV plus MS-T, a cell-free cytoplasmic extract is prepared which will support VSV mRNA synthesis and the synthesis of the 42S wild-type or 19S MS-T genome RN...
متن کاملInitiation of RNA synthesis in vitro by vesicular stomatitis virus. Role of ATP.
The ATP requirement during RNA transcription by the RNA-dependent RNA polymerase associated with purified vesicular stomatitis virus was studied during chain initiation and chain elongation steps. Initiation of transcription in vitro has an apparent Km for ATP of approximately 500 micron, whereas the apparent Km for ATP during chain elongation is almost identical with those for the other three ...
متن کاملMechanism of RNA synthesis initiation by the vesicular stomatitis virus polymerase.
The minimal RNA synthesis machinery of non-segmented negative-strand RNA viruses comprises a genomic RNA encased within a nucleocapsid protein (N-RNA), and associated with the RNA-dependent RNA polymerase (RdRP). The RdRP is contained within a viral large (L) protein, which associates with N-RNA through a phosphoprotein (P). Here, we define that vesicular stomatitis virus L initiates synthesis ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.20.7031